Simpler Purification: No cell lysis needed, resulting in fewer contaminants and more efficient purification.

Higher Protein Quality: Secreted cytokines are more likely to be properly folded and modified, enhancing biological activity.
Improved Yields: Soluble cytokines in supernatants avoid inclusion body formation, leading to better functional yields.
Lower Endotoxin Risk: Eukaryotic systems like CHO or HEK293 cells reduce endotoxin contamination, crucial for therapeutic applications.
Easier Scalability: Supernatant purification is simpler to scale compared to intracellular methods.
Cost-Effective: Fewer purification steps and reduced need for lysis reagents lower overall costs.